| Mass spectrometer | Bruker, Autoflex LRF |
| MS source | MALDI |
| Detection orientations | Linear mode (best for masses > 5 kDa) Reflectron mode (best for masses < 5 kDa) |
| Resolving power | 15,000 |
| Mass accuracy | 20-50 ppm |
| MS level available | MS1 |
Matrix-Assisted Laser Desorption Ionization (MALDI) is a soft ionization technique. The sample is mixed with a matrix (small organic molecule that absorbs at the laser’s wavelength) in a common solution. 1 uL of the sample is spotted on the plate and allowed to dry, which causes the matrix to crystallize with the analyte is embedded in the matrix. Here we use a nitrogen laser (335 nm) on the sample spot which causes the desportion and ionzation event. The ions are analyzed by time of flight mass analyzer. The Autoflex offers high resolution (reflectron mode for compounds less than 5,000 Da) and low resolution (linear mode for compounds greater than 5,000 Da). MALDI is ideal for polymers, peptides, intact proteins and a wide variety of compounds.
Standard applications
- High resolution, large mass analysis
- Polymer, peptides, intact protein, and oligomer mass analysis
Available for self-operation!