| Chromatography | Thermo, Dionex Ultimate 3000 RSLCnano UPLC |
| Mass spectrometer | Bruker, timsTOF Pro2 |
| Additional separation | Trapped Ion Mobility (tims) |
| MS source | ESI |
| Resolving power | 60,000 |
| Mass accuracy | < 2 ppm |
| MS level available | MS1, MS2, CCS |
Ion mobility MS for “4D Omics” analysis of extreme complex mixtures and ultra sensitivity. Trapped ion-mobility combined with parallel accumulation serial fragmentation (PASEF) allows for fragmentation of 12 precursors within 100 ms. Accumulation of precursor ions also leads to a significant increase in S/N ratio. Equipped with a Dionex UPLC that can run both in UPLC and HPLC mode. We also have the ability to switch between nano-flow and capillary-flow.
Standard applications
- Ultra-high resolution, accurate mass (UHRMS) analysis
- Accurate mass with mobility separation
- For isobaric analytes having the same ion mass (ie, molecular weight) but different confirmations (ie, molecular shapes)
- 4-D compound evaluation: LC retention time, tims mobility (1/k0, CCS), ion mass, fragmentation pattern
- Targeted, semi-targeted, or untargeted 3-D LC-MS/MS metabolomic, lipidomic, or proteomic analysis
- Targeted, semi-targeted, or untargeted 4-D IMS-LC-MS/MS metabolomic, lipidomic, or proteomic analysis
- Intact protein