| Chromatography | Agilent, 1100 HPLC |
| Mass spectrometer | Agilent, 6230 LCMS |
| Additional detectors | Agilent, DAD UV (190-400 nm) |
| MS source(s) | ESI DART |
| Resolving power | 12,000 |
| Mass accuracy | ≤ 5 ppm |
| MS level available | MS1 |
Used primarily for ESI or DART high-resolution (~12,000) analysis for accurate mass measurements (within 5 ppm). ESI is well suited for polar molecules soluble in methanol, water, or acetonitrile and DART is best for non-polar molecules. Samples for DART can be measured neat as a solid or liquid or solubilized in a solvent. For ESI, samples are prepared in HPLC vials and injected through an auto-sampler for high-throughput analysis. An Agilent 1100 HPLC for LC-MS applications is also available for this instrument. For comparative purposes, an in-line UV detector is also available for LC-UV-MS analyses.
Standard applications
- High-resolution, accurate mass (HRMS) analysis
- Accurate mass LC-MS
- Intact protein
Available for self-operation!