| Chromatography | Thermo, Dionex Ultimate 3000 RSLCnano UPLC |
| Mass spectrometer | Thermo, Q Exactive HF Orbitrap |
| Ionization source | ESI |
| Resolving power (selective) | 60,000 120,000 160,000 |
| Mass accuracy | < 2 ppm |
| MS level available | MS1 and MS2 |
The Q Exactive Plus LC-MS/MS is a tandem mass spectrometer that combines a quadrupole mass analyzer for precursor selection with a high-resolution/high mass accuracy Orbitrap mass analyzer. The complete system includes Protein Mode for enhanced analysis of intact proteins and protein complexes, an UltiMate 3000 RSLCnano LC system, a Nanospray Flex Ion Source, ability to change the system between capillary and nano flow, Biopharma Finder 1.0, and Proteome Discoverer 2.1 Base and Quantitation.
The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders.
NIH Funded (NIH S10 OD021758-01A1) mass spectrometer.
Standard applications
- Targeted, semi-targeted, or untargeted proteomic LC-MS
- Intact protein