Omics – Proteomics – Lipidomics – Metabalomics

• Proteomics, Metabolomics and Lipidomics studies the large-scale changes in small molecules, lipids or proteins from perturbations of biological systems.
• Important in environmental sciences, pharmacology, nutrition, biophysics, cell biology, pathology and disease diagnostics
• Significant challenges in sample preparation, chromatography, ionization, and mass analyses and bioinformatics.

Liquid chromatography (LC) and mass spectrometry (MS) is  used to separate and analyze polar compounds from a complex mixture. Quantitative comparison between experimental groups can reveal changes in abundances in complex mixtures from tissue, cells, plasma or other extracts.  Typically polar molecules are separated on a C18 HPLC column with water/methanol or water acetonitrile mobile phases. If the LC is coupled with tandem mass spectrometry (MS/MS) the compounds eluting from the column can be fragmented and provides structural/sequence information.