Submitting Samples and Rates
Instructions for Submitting Samples to the Mass Spectrometry Facility
- If you do not know what kind of service you need please contact Dr. Kari Basso .
- Download/save to fill out a service request form (please type the information directly into the form): To type directly into the form you can save the file and when you open it in Adobe Reader you should have the ability to type in the forms. Or you can open it from the web using Adobe Acrobat Pro and type in directly.
- ON CAMPUS Please download/save if you are affiliated with University of Florida.
- OFF CAMPUS Please download/save if you are an Off-Campus user.
- SELF OP Please download if you are a Self-OP user.
- You have the ability to insert your structure directly as a image (jpeg tiff etc). Download the Service Request Form. Save your Chem Draw as an image file. Click on the Image icon and and follow the guidance in choosing the image to insert. If you only have Adobe Reader you will be able to type in the form fields but not insert an image. You will need to attach your image on a separate sheet.
- Please fill the form out completely and with as much information as possible about your sample. Use the comment section to provide additional requests or sample information. Y
- You may use one form for several samples submitted at the same time if you prefer. After you fill it out, you can save the file by selecting “File”, “Save As”.
- Print the form when you are done and bring it with your sample. You can also email it to Dr. Kari Basso.
- Bring the printed form and the sample(s) to the UF Chemistry Laboratory Building (CLB), room 101, between 9 am and 4 pm weekdays. Please enter your information at the computer station and the entrance of the lab.
- If you are mailing the samples, please use Fed-Ex or UPS as a carrier, do not mail for weekend delivery. Ship the form and samples to:
Dr. Kari Basso
1680 Union Rd
126 Sisler Hall/Department of Chemistry
Gainesville FL 32611 - All data is stored electronically. We will email results to you.
- Samples will be discarded unless requested to return.
- Please read
Minimizing Contamination for Successful MS Results
Rates*
| Unit | UF | Non-UF University/Govt | Industry | Description | |
|---|---|---|---|---|---|
| Ultra-high resolution accurate mass (Impact or Orbitrap) | Sample | $55 | $65 | $165 | Sub-ppm accuracy. MS/MS for structure determination. Can be used with Smart Formula to identify molecular formula for true unknowns. Molecular formula matching for molecules < 1000 amu. |
| Ultra-high resolution Ion Mobility (timsTOF Pro II) | Sample | $75 | $90 | $215 | Direct infusion ion mobility for isomer identification |
| Nominal mass or molecular formula matching (Agilent, MALDI) | Sample | $45 | $55 | $135 | Molecular formula matching to predicted molecular formula to within 5 ppm for masses less than 1000 amu |
| Calibration Curve | Project | $330 | $400 | $825 | 5-point calibration curve for targeted quantitation |
| Low-resolution GC-MS Standard Gradient | Injection | $45 | $55 | $135 | Standard GC-MS on the ISQ with a 10 minute 30-minute gradient |
| High-resolution GC-MS/DIP | Injection | $60 | $70 | $165 | High-resolution GC-MS on the Orbitrap with a 10 minute 30-minute gradient |
| High-Resolution GC-MS/DIP > 10 samples | Injection | $55 | $65 | $165 | Bulk rate for high-throughput projects |
| High Resolution GC-MS Long Gradient | Injection | $80 | $90 | $240 | Greater than 30-minute gradient for complex separations. |
| LC-MS or GC-MS method development | Project | $425 | $495 | $770 | Method development for targeted LC-MS projects using standards. |
| Low resolution LC-MS/MS (LTQ, Quad) | Injection | $75 | $90 | $215 | LC-MS/MS on one of the low-resolution instruments |
| Low resolution LC-MS/MS > 10 samples (LTQ, Quad) | Injection | $55 | $65 | $165 | Bulk rate for high-throughput projects |
| Medium Resolution LC-MS (TOF) | Injection | $100 | $125 | $300 | LC-MS with DAD on the TOF added if desired |
| Medium Resolution LC-MS (TOF) > 10 samples | Injection | $85 | $100 | $215 | Bulk rate for high-throughput projects |
| High resolution LC-MS (Impact, Orbitrap) | Injection | $150 | $165 | $385 | LC-MS or LC-MS/MS on high resolution instruments |
| High resolution LC-MS (Impact, Orbitrap) > 10 samples | Injection | $130 | $155 | $315 | Bulk rate for high-throughput projects |
| High resolution LC-Ion-Mobility MS (timsTOF Pro II) | Injection | $185 | $220 | $545 | LC-IMS-MS or LC-IMS-MS/MS on timsTOF Pro |
| High resolution LC-MS (timsTOF Pro II) > 10 samples | Injection | $150 | $175 | $440 | Bulk rate for high-throughput projects |
| Statistical Analysis and Bioinformatics | Project | $250 | $300 | $600 | Quantitative and statistical analysis and metabolite identification |
| Training/Staff Time | Hour | $45 | $55 | $132 | |
| Self-operation MALDI | Hour | $20 | $30 | $60 | Billed in 30-minute increments |
| Self-operation Flow injection on Agilent or QTOF | Hour | $90 | $105 | $265 | Billed in 30-minute increments |
| Self-Operation LC-MS or GC-MS | Hour | $35 | $40 | $105 | Billed in 30 min-increments |
| 1D SDS PAGE and coomassie stain | 10 well Gel | $72 | $88 | $215 | Up to 8 samples (8 wells) |
| Albumin removal/phosphopeptide enrichment | Sample | $60 | $75 | $180 | Sample preparation |
| Cell lysis/protein/metabolite/lipid extraction | Sample | $60 | $75 | $180 | Sample preparation |
| Protein quantitation (Qubit) or total lipid SPVA | Project | $90 | $110 | $265 | Quantitation analysis for normalization |
| Sample Fractionation by pI | Sample | $90 | $110 | $265 | Pre-fractionate complex protein mixtures by pI to detect more low abundant proteins |
| Standard Protein Sequencing (solution, on-bead, or gel band) | Sample | $140 | $180 | $360 | Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE |
| Standard Protein Sequencing > 10 samples | Sample | $120 | $145 | $300 | Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE |
| TMT-ProÔ Labeling | Sample | $140 | $165 | $395 | TMT Labeling up to 18 samples for ultimate proteomic quantitation |
| Complex Protein Sequencing | Sample | $220 | $265 | $550 | Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE |
| Complex Protein Sequencing > 10 samples | Sample | $200 | $240 | $495 | Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE |
| TMT Ultra Complex Protein Sequencing | Sample | $350 | $385 | $1000 | Digestion, LC-MS/MS 6-hour LC-MS gradient for TMT samples |
| Additional Enzyme | Sample | $60 | $75 | $165 | Chymotrypsin, Glu-C etc. |
| Desalting Protein/DNA | Sample | $35 | $45 | $100 | For intact mass measurement |
| PTM or quantitative analysis using Proteome Discoverer, Scaffold, Peaks, or Mascot | Sample | $250 | $300 | $600 | Quantitative analysis with statistical analysis and/or post-translational modification analysis |
* Rush charges are 3x standard published rates.