Submitting Samples and Rates

Instructions for Submitting Samples to the Mass Spectrometry Facility

  1. If you do not know what kind of service you need please contact Dr. Kari Basso .
  2. Download/save to fill out a service request form (please type the information directly into the form): To type directly into the form you can save the file and when you open it in Adobe Reader you should have the ability to type in the forms. Or you can open it from the web using Adobe Acrobat Pro and type in directly.
  3. ON CAMPUS    Please download/save if you are affiliated with University of Florida.
  4. OFF CAMPUS Please download/save if you are an Off-Campus user.
  5. SELF OP  Please download if you are a Self-OP user.
  6. You have the ability to insert your structure directly as a image (jpeg tiff etc). Download the Service Request Form.  Save your Chem Draw as an image file. Click on the Image icon and and follow the guidance in choosing the image to insert. If you only have Adobe Reader you will be able to type in the form fields but not insert an image. You will need to attach your image on a separate sheet.
  7. Please fill the form out completely and with as much information as possible about your sample. Use the comment section to provide additional requests or sample information.  Y
  8. You may use one form for several samples submitted at the same time if you prefer. After you fill it out, you can save the file by selecting “File”, “Save As”.
  9. Print the form when you are done and bring it with your sample.  You can also email it to Dr. Kari Basso.
  10. Bring the printed form and the sample(s) to the UF Chemistry Laboratory Building (CLB), room 101, between 9 am and 4 pm weekdays.  Please enter your information at the computer station and the entrance of the lab.
  11. If you are mailing the samples, please use Fed-Ex or UPS as a carrier, do not mail for weekend delivery.   Ship the form and samples to:

    Dr. Kari Basso
    1680 Union Rd
    126 Sisler Hall/Department of Chemistry
     Gainesville FL 32611

  12. All data is stored electronically. We will email results to you.
  13. Samples will be discarded unless requested to return.
  14. Please read

Minimizing Contamination for Successful MS Results

Rates

*Rush Charges are 3 x standard published rates

 

Unit

UF

Non-UF University/Govt

Industry

Description

Molecular Weight/Chemical Formula Determination

          

Ultra-high resolution accurate mass (Impact or Orbitrap)

Sample

$50

$60

$150

Sub-ppm accuracy.  MS/MS for structure determination.  Can be used with Smart Formula to identify molecular formula for true unknowns.  Molecular formula matching for molecules < 1000 amu.

Ultra-high resolution Ion Mobility (timsTOF Pro II)

Sample

$65

$80

$195

Direct infusion ion mobility for isomer idenetification

Nominal mass or molecular formula matching (Agilent, MALDI)

Sample

$40

$50

$120

Molecular formula matching to predicted molecular formula to within 5 ppm for masses less than 1000 amu (Agilent, MALDI) or nominal mass MS/MS (LTQ)

Lipidomics/Metabolomics/Small Molecule

 

          
Calibration Curve Project $300 $360 $750 5 point calibration curve for targeted quantitation

GC-MS Standard Gradient/DIP

Injection

$40

$50

$120

Standard GC-MS with a 10 minute – 30 minute gradient

GC-MS Long Gradient

Injection

$55

$65

$165

GC-MS Gradients longer than 30 minutes

LC-MS method development

Project

$385

$450

$700

Method development for LC-MS projects using standards.

Low resolution LC-MS/MS (LTQ, Quad)

Injection

$65

$80

$195

LC-MS/MS on one of the low-resolution instruments

Low resolution LC-MS/MS > 10 samples (LTQ, Quad)

Injection

$50

$60

$150

Bulk rate for high-throughput projects

Medium Resolution LC-MS (TOF)

Injection

$90

$110

$270

LC-MS with DAD on the TOF added if desired

Medium Resolution LC-MS (TOF) > 10 samples

Injection

$75

$90

$195

Bulk rate for high-throughput projects

High resolution LC-MS (Impact, Orbitrap)

Injection

$135

$160

$350

LC-MS or LC-MS/MS on high resolution instruments

High resolution LC-MS (Impact, Orbitrap) > 10 samples

Injection

$115

$140

$285

Bulk rate for high-throughput projects

High resolution LC-Ion-Mobility MS (timsTOF Pro II)

Injection

$165

$200

$495

LC-IMS-MS or LC-IMS-MS/MS on timsTOF Pro

High resolution LC-MS (timsTOF Pro II) > 10 samples

Injection

$135

$160

$400

Bulk rate for high-throughput projects

Statistical Analysis and Bioinformatics

Project

$220

$270

$550

Quantitative and statistical analysis and metabolite identification

Self-Operation

 

          

Training/Staff Time

Hour

$40

$50

$120

  

Self-operation MALDI

Hour

$18

$25

$55

Billed in 30-minute increments

Self-operation Flow injection on Agilent or QTOF

Hour

$80

$95

$240

Billed in 30-minute increments

Proteomics

 

          

1D SDS PAGE and coomassie stain

10 well Gel

$65

$80

$195

Up to 10 samples (10 wells)

Albumin removal/phosphopeptide enrichment

Sample

$55

$65

$165

Sample preparation

Cell lysis/protein/metabolite/lipid extraction

Sample

$55

$65

$165

Sample preparation

Protein quantitation (Qubit) or total lipid SPVA

Project

$80

$100

$240

Sample preparation

Sample Fractionation by pI

Sample

$80

$100

$240

Pre-fractionate complex protein mixtures by pI to detect more low abundant proteins

Standard Protein Sequencing (solution, on-bead, or gel band)

Sample

$130

$160

$325

Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE

Standard Protein Sequencing  > 10 samples

Sample

$110

$130

$275

Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE

TMT-ProTM Labeling

Sample

$150

$180

$360

TMT Labeling up to 18 samples for ultimate proteomic quantitation

Complex Protein Sequencing

Sample

$200

$240

$500

Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE

Complex Protein Sequencing > 10 samples

Sample

$180

$220

$450

Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE

Additional Enzyme

Sample

$55

$65

$150

Chymotrypsin, Glu-C etc. 

Desalting Protein/DNA

Sample

$30

$40

$90

For intact mass measurement

PTM or quantitative analysis using Proteome Discoverer, Scaffold, Peaks, or Mascot

Sample

$220

$270

$550

Quantitative analysis with statistical analysis and/or post-translational modification analysis

TMT-LC MS/MS 6 hour

gradient

 sample $300 $360 $900  
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           
           

* Rush charges are 3x standard published rates.