Instructions for submitting samples
- If you do not know what kind of service you need, please contact Dr. Kari Basso.
- Download/save to fill out a service request form (please type the information directly into the form):
- To type directly into the form, save the file and when you open it in Adobe Reader you should have the ability to type in the forms.
- Or you can open it from the web using Adobe Acrobat Pro and type in directly.
- ON CAMPUS – Please download/save if you are affiliated with University of Florida.
- OFF CAMPUS – Please download/save if you are an Off-Campus user.
- SELF OP – Please download/save if you are a Self-Op user.
- Attach or insert (jpeg, tiff, etc.) your structure with the service request form.
- Download the service request form.
- Save your Chem Draw structure as an image file.
- Click on the image icon and follow the guidance in choosing the image to insert.
- If you cannot insert an image directly, attach your structure on a separate sheet.
- Please fill the form out completely and with as much information as possible about your sample.
- Use the comment section to provide additional requests or sample information, such as sample storage conditions (e.g., light sensitive, store in refrigerator).
- You may use ONE form for several samples being submitted at the same time.
- Use a separate sheet to provide the sample name, structure, chemical formula, and molecular weight for all samples being submitted.
- Save the file by selecting “File” > “Save As”.
- Print the form when you are done and bring it with your sample. You can also email it to Dr. Kari Basso. form when
- Bring the printed form and the sample(s) to the UF Chemistry Laboratory Building (CLB), room 101, between 9 am and 4 pm weekdays. Please enter your information at the computer station and the entrance of the lab.
- If you are mailing the samples, please use Fed-Ex or UPS as a carrier.
- Do not mail for weekend delivery.
- Ship the form and samples to:
Dr. Kari Basso
1680 Union Rd
126 Sisler Hall/Department of Chemistry
Gainesville FL 32611
- All data is stored electronically. We will email results to you.
- Samples will be discarded unless sample return requested.
- Please read!!!
Rates
| Unit | UF | Non-UF University/Govt | Industry | Description | |
|---|---|---|---|---|---|
| Ultra-high resolution accurate mass (Impact or Orbitrap) | Sample | $55 | $65 | $165 | Sub-ppm accuracy. MS/MS for structure determination. Can be used with Smart Formula to identify molecular formula for true unknowns. Molecular formula matching for molecules < 1000 amu. |
| Ultra-high resolution Ion Mobility (timsTOF Pro II) | Sample | $75 | $90 | $215 | Direct infusion ion mobility for isomer identification |
| Nominal mass or molecular formula matching (Agilent, MALDI) | Sample | $45 | $55 | $135 | Molecular formula matching to predicted molecular formula to within 5 ppm for masses less than 1000 amu |
| Calibration Curve | Project | $330 | $400 | $825 | 5-point calibration curve for targeted quantitation |
| Low-resolution GC-MS Standard Gradient | Injection | $45 | $55 | $135 | Standard GC-MS on the ISQ with a 10 minute 30-minute gradient |
| High-resolution GC-MS/DIP | Injection | $60 | $70 | $165 | High-resolution GC-MS on the Orbitrap with a 10 minute 30-minute gradient |
| High-Resolution GC-MS/DIP > 10 samples | Injection | $55 | $65 | $150 | Bulk rate for high-throughput projects |
| High Resolution GC-MS Long Gradient | Injection | $80 | $90 | $240 | Greater than 30-minute gradient for complex separations. |
| LC-MS or GC-MS method development | Project | $425 | $495 | $770 | Method development for targeted LC-MS projects using standards. |
| Low resolution LC-MS/MS (LTQ, Quad) | Injection | $75 | $90 | $215 | LC-MS/MS on one of the low-resolution instruments |
| Low resolution LC-MS/MS > 10 samples (LTQ, Quad) | Injection | $55 | $65 | $165 | Bulk rate for high-throughput projects |
| Medium Resolution LC-MS (TOF) | Injection | $100 | $125 | $300 | LC-MS with DAD on the TOF added if desired |
| Medium Resolution LC-MS (TOF) > 10 samples | Injection | $85 | $100 | $215 | Bulk rate for high-throughput projects |
| High resolution LC-MS (Impact, Orbitrap) | Injection | $150 | $165 | $385 | LC-MS or LC-MS/MS on high resolution instruments |
| High resolution LC-MS (Impact, Orbitrap) > 10 samples | Injection | $135 | $155 | $315 | Bulk rate for high-throughput projects |
| High resolution LC-Ion-Mobility MS (timsTOF Pro II) | Injection | $185 | $220 | $545 | LC-IMS-MS or LC-IMS-MS/MS on timsTOF Pro |
| High resolution LC-MS (timsTOF Pro II) > 10 samples | Injection | $165 | $185 | $440 | Bulk rate for high-throughput projects |
| Statistical Analysis and Bioinformatics | Project | $250 | $300 | $600 | Quantitative and statistical analysis and metabolite identification |
| Training/Staff Time | Hour | $45 | $55 | $132 | |
| Self-operation MALDI | Hour | $20 | $30 | $60 | Billed in 30-minute increments |
| Self-operation Flow injection on Agilent or QTOF | Hour | $90 | $105 | $265 | Billed in 30-minute increments |
| Self-Operation LC-MS or GC-MS | Hour | $35 | $40 | $105 | Billed in 30 min-increments |
| 1D SDS PAGE and coomassie stain | 10 well Gel | $72 | $88 | $215 | Up to 8 samples (8 wells) |
| Albumin removal/phosphopeptide enrichment | Sample | $60 | $75 | $180 | Sample preparation |
| Cell lysis/protein/metabolite/lipid extraction | Sample | $60 | $75 | $180 | Sample preparation |
| Protein quantitation (Qubit) or total lipid SPVA | Project | $90 | $110 | $265 | Quantitation analysis for normalization |
| Sample Fractionation by pI | Sample | $90 | $110 | $265 | Pre-fractionate complex protein mixtures by pI to detect more low abundant proteins |
| Standard Protein Sequencing (solution, on-bead, or gel band) | Sample | $140 | $180 | $360 | Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE |
| Standard Protein Sequencing > 10 samples | Sample | $120 | $145 | $300 | Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE |
| TMT-ProÔ Labeling | Sample | $140 | $165 | $395 | TMT Labeling up to 18 samples for ultimate proteomic quantitation |
| Complex Protein Sequencing | Sample | $220 | $265 | $550 | Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE |
| Complex Protein Sequencing > 10 samples | Sample | $200 | $240 | $495 | Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE |
| TMT Ultra Complex Protein Sequencing | Sample | $350 | $385 | $1000 | Digestion, LC-MS/MS 6-hour LC-MS gradient for TMT samples |
| Additional Enzyme | Sample | $60 | $75 | $165 | Chymotrypsin, Glu-C etc. |
| Desalting Protein/DNA | Sample | $35 | $45 | $100 | For intact mass measurement |
| PTM or quantitative analysis using Proteome Discoverer, Scaffold, Peaks, or Mascot | Sample | $250 | $300 | $600 | Quantitative analysis with statistical analysis and/or post-translational modification analysis |
*Rush charges are 3x standard published rates