Submissions and Rates

Submission instructions
Rates

Instructions for submitting samples

1. If you do not know what kind of service you need, please contact Dr. Kari Basso.
2. SELF OP – Please download/save if you are a Self-Op user.
3. Please fill the form out completely and with as much information as possible about your sample.
   • Use the comment section to provide additional requests or sample information, such as sample storage conditions (e.g., light sensitive, store in refrigerator).
4. You may use ONE form for several samples being submitted at the same time.
   • Use a separate sheet to provide the sample name, structure, chemical formula, and molecular weight for all samples being submitted.
5. Print the form when you are done and bring it with your sample and bring structures of your molecules if you are requesting accurate mass analysis . 
6. Bring the printed form and the sample(s) to the UF Chemistry Laboratory Building (CLB), room 101, between 9 am and 4 pm weekdays.  Please enter your information at the computer station and the entrance of the lab.
7. If you are mailing the samples, please use Fed-Ex or UPS as a carrier.
   • Do not mail for weekend delivery.  
   • Ship the form and samples to:

13. All data is stored electronically. We will email results to you.
14. Samples will be discarded unless sample return requested.
15. Please read!!!
    • Minimizing contamination for successful MS results

Rates

	Unit	University of Florida	Non-UF University/Govt	Industry	Description
1D SDS PAGE and coomassie stain	10 well Gel	$72	$88	$215	Up to 8 samples (8 wells)
Training/Staff Time	Hour	$45	$55	$132
Self-operation MALDI	Hour	$20	$30	$60	Billed in 30-minute increments
Self-operation Flow injection on Agilent or QTOF	Hour	$90	$105	$265	Billed in 30-minute increments
Self-Operation LC-MS or GC-MS	Hour	$35	$40	$105	Billed in 30 min-increments
Low-resolution GC-MS Standard Gradient	Injection	$45	$55	$135	Standard GC-MS on the ISQ with a 10 minute – 30-minute gradient
High-resolution GC-MS/DIP	Injection	$60	$70	$165	High-resolution GC-MS on the Orbitrap with a 10 minute – 30-minute gradient
High-Resolution GC-MS/DIP > 10 samples	Injection	$55	$65	$150	Bulk rate for high-throughput projects
High Resolution GC-MS Long Gradient	Injection	$80	$90	$240	Greater than 30-minute gradient for complex separations.
Low resolution LC-MS/MS (LTQ, Quad)	Injection	$75	$90	$215	LC-MS/MS on one of the low-resolution instruments
Low resolution LC-MS/MS > 10 samples (LTQ, Quad)	Injection	$55	$65	$165	Bulk rate for high-throughput projects
Medium Resolution LC-MS (TOF)	Injection	$100	$125	$300	LC-MS with DAD on the TOF added if desired
Medium Resolution LC-MS (TOF) > 10 samples	Injection	$85	$100	$215	Bulk rate for high-throughput projects
High resolution LC-MS (Impact, Orbitrap)	Injection	$150	$165	$385	LC-MS or LC-MS/MS on high resolution instruments
High resolution LC-MS (Impact, Orbitrap) > 10 samples	Injection	$135	$155	$315	Bulk rate for high-throughput projects
High resolution LC-Ion-Mobility MS (timsTOF Pro II)	Injection	$185	$220	$545	LC-IMS-MS or LC-IMS-MS/MS on timsTOF Pro
High resolution LC-MS (timsTOF Pro II) > 10 samples	Injection	$165	$185	$440	Bulk rate for high-throughput projects
Calibration Curve	Project	$330	$400	$825	5-point calibration curve for targeted quantitation
LC-MS or GC-MS method development	Project	$425	$495	$770	Method development for targeted LC-MS projects using standards.
Statistical Analysis and Bioinformatics	Project	$250	$300	$600	Quantitative and statistical analysis and metabolite identification
Protein quantitation (Qubit) or total lipid SPVA	Project	$90	$110	$265	Quantitation analysis for normalization
Ultra-high resolution accurate mass (Impact or Orbitrap)	Sample	$55	$65	$165	Sub-ppm accuracy. MS/MS for structure determination. Can be used with Smart Formula to identify molecular formula for true unknowns. Molecular formula matching for molecules < 1000 amu.
Ultra-high resolution Ion Mobility (timsTOF Pro II)	Sample	$75	$90	$215	Direct infusion ion mobility for isomer identification
Nominal mass or molecular formula matching (Agilent, MALDI)	Sample	$45	$55	$135	Molecular formula matching to predicted molecular formula to within 5 ppm for masses less than 1000 amu
Albumin removal/phosphopeptide enrichment	Sample	$60	$75	$180	Sample preparation
Cell lysis/protein/metabolite/lipid extraction	Sample	$60	$75	$180	Sample preparation
Sample Fractionation by pI	Sample	$90	$110	$265	Pre-fractionate complex protein mixtures by pI to detect more low abundant proteins
Standard Protein Sequencing (solution, on-bead, or gel band)	Sample	$140	$180	$360	Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE
Standard Protein Sequencing > 10 samples	Sample	$120	$145	$300	Digestion LC-MS/MS and bioinformatics 1 Hr gradient on the QE
TMT-ProÔ Labeling	Sample	$140	$165	$395	TMT Labeling up to 18 samples for ultimate proteomic quantitation
Complex Protein Sequencing	Sample	$220	$265	$550	Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE
Complex Protein Sequencing > 10 samples	Sample	$200	$240	$495	Digestion, LC-MS/MS and bioinformatics 3 hour gradient on the QE
TMT Ultra Complex Protein Sequencing	Sample	$350	$385	$1000	Digestion, LC-MS/MS 6-hour LC-MS gradient for TMT samples
Additional Enzyme	Sample	$60	$75	$165	Chymotrypsin, Glu-C etc.
Desalting Protein/DNA	Sample	$35	$45	$100	For intact mass measurement
PTM or quantitative analysis using Proteome Discoverer, Scaffold, Peaks, or Mascot	Sample	$250	$300	$600	Quantitative analysis with statistical analysis and/or post-translational modification analysis

*Rush charges are 3x standard published rates