Omics – Proteomics – Lipidomics – Metabalomics
Liquid chromatography (LC) and mass spectrometry (MS) is used to separate and analyze polar compounds from a complex mixture. Quantitative comparison between experimental groups can reveal changes in abundances in complex mixtures from tissue, cells, plasma or other extracts. Typically polar molecules are separated on a C18 HPLC column with water/methanol or water acetonitrile mobile phases. If the LC is coupled with tandem mass spectrometry (MS/MS) the compounds eluting from the column can be fragmented and provides structural/sequence information.