Submitting Samples and Rates

Instructions for Submitting Samples to the Mass Spectrometry Facility

  1. If you do now know what kind of service you need please contact Dr. Kari Basso .
  2. Download/save to fill out a service request form (please type the information directly into the form): To type directly into the form you can save the file and when you open it in Adobe Reader you should have the ability to type in the forms. Or you can open it from the web using Adobe Acrobat Pro and type in directly.
  3. ON CAMPUS    Please download/save if you are affiliated with University of Florida.
  4. OFF CAMPUS Please download/save if you are an Off-Campus user.
  5. SELF OP  Please download if you are a Self-OP user.
  6. You have the ability to insert your structure directly as a image (jpeg tiff etc). Download the Service Request Form.  Save your Chem Draw as an image file. Click on the Image icon and and follow the guidance in choosing the image to insert. If you only have Adobe Reader you will be able to type in the form fields but not insert an image. You will need to attach your image on a separate sheet.
  7. Please fill the form out completely and with as much information as possible about your sample. You may use one form for several samples submitted at the same time if you prefer. After you fill it out, you can save the file by selecting “File”, “Save As”.
  8. Print the form when you are done or email it to Dr Manasi Kamat mangaonkar@chem.ufl.edu
  9. Bring the printed form and the sample(s) to the UF Chemistry Laboratory Building (CLB), room 101, between 9 am and 4 pm weekdays.
  10. If you are mailing the samples, please use Fed-Ex or UPS as a carrier and ship the form and samples to:

    Dr. Kari Basso
    1680 Union Rd
    126 Sisler Hall/Department of Chemistry
     Gainesville FL 32611

  11. All data is stored electronically. We will email results to you.
  12. Samples will be discarded unless requested to return.
  13. Please read

Minimizing Contamination for Successful MS Results

Rates

*Rush Charges are 3 x standard published rates

  Unit UF Non-UF University/Govt Industry Description
Molecular Weight/Chemical Formula Determination
         

Ultra-high resolution accurate mass (Impact)

Sample

$50 $60 $150 Sub-ppm accuracy.   MS/MS for structure determination.   Can be used with Smart Formula to identify molecular formula for true unknowns.
Nominal mass or molecular formula matching (Agilent, MALDI) Sample $40 $50 $115 Molecular formula matching to predicted molecular formula to within 10 ppm (Agilent, MALDI) or nominal mass MS/MS (LTQ-XL)

Metabolomics/Small Molecule

         

GC-MS Method Development

Project $165 $200 $495 Method development for GC-MS projects using standards.
GC-MS or DIP Injection $35 $45 $105 Standard GC-MS
GC-MS > 10 samples Injection $30 $35 $70 Bulk rate for higher-throughput projects
LC-MS method development Project $385 $450 $700 Method development for LC-MS projects using standards.
Low resolution LC-MS (LTQ, Quad) Injection $65 $80 $195 LC-MS or LC-MS/MS on the LTQ-XL with a standard gradient. SIM scan, full scan, data-dependent scans available on the LTQ-XL. 2.1 mm column recommended

Low resolution LC-MS > 10 samples (LTQ, Quad)

Injection

$50

$60

$150

Bulk rate for higher-throughput projects.

Medium Resolution LC-MS (Agilent TOF)

Injection $90 $120 $270 Medium resolution (15,000 – 20,000) on the TOF.  UV detection availalbe.
Medium Resolution LC-MS > 10 samples (Aglient TOF) Injection $65 $75 $195 Bulk rate for higher-throughput projects.
Ultra-High resolution LC-MS (Impact, Orbitrap) Injection $135 $190 $405 Ultra-high resolution (less than 2 ppm mass accuracy) capillary flow LC-MS (300 um column 5 ul/min flow rate). Nano-flow is available.
Ultra-High resolution LC-MS (Impact) > 10 samples Injection $115 $170 $345 Bulk rate for higher-throughput projects.

Data Analysis, Statistical Analysis and Bioinformatics Metaboscape

Project $220 $350 $660 Quantitative Analysis including PCA, volcano, t-tests. Library searching

Self-Operation

         
Training/Staff Time Hour $40 $50 $120 Training, sample prep, complex data processing, bioinformatics.
Self-operation MALDI Hour $18 $25 $55 Requires training. Billed at 30 minute minimum.
Self-Operation Flow injection on Agilent TOF or QTOF Hour $80 $95 $240 Requires training.  Billed at 30 minute minimum.

Proteomics

         
1D SDS PAGE and coomassie stain 10 well Gel $65 $75 $195 1D-SDS PAGE, up to 12 samples per gel. Standard coomassie stain.
Albumin removal/phosphopeptide enrichment Sample $55 $70 $165 Sample preparation
Cell lysis/protein/lipid/metabolite extraction Sample $55 $70 $165 Sample preparation
Total Protein quantitation (Qubit) or total Lipid SPVA Project $80 $100 $240 Sample preparation
Standard Protein Sequencing (solution or gel band) 90 minute gradient Sample $130 $160 $390 Includes enzyme digestion, LC-MS/MS on QE Exactive and bioinformatics.
Standard Protein Sequencing > 10 samples Sample $110 $130 $330 Bulk rate for higher-throughput projects.

Complex Protein Sequencing (whole cell lysates) 150 minute gradient

Sample $200 $240 $600  
Complex Protein Sequencing > 10 samples Sample $180 $200 $540 Digestion, LC-MS/MS and bioinformatics 2 – 5 hour gradient on the QE

PTM or quantitative analysis, statistical analysis

Proteome Discoverer, Matrix Science or Scaffold

Sample $220 $350 $660 In-depth bioinformatic analysis to determine protein modifications or protein quantitation.
Additional enzyme digestion (chymotrypsin, AspN, etc) Sample $55 $65 $150 Additional enzyme digestion beyond the one included in the standard protein ID.
Desalting Protein/DNA Sample $30 $40 $85  

* Rush charges are 3x standard published rates.